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2 edition of development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles found in the catalog.

development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles

F. Elizabeth Gibson

development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles

by F. Elizabeth Gibson

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Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1991.

Statementby F. Elizabeth Gibson.
ID Numbers
Open LibraryOL19657940M

  Genetic system development in Sulfolobus has been underway for over a decade, but remains a research focus. Thermus thermophilus First isolated from a Japanese hot spring in , Thermus thermophilus is an aerobic bacterium that grows best between 70°C (Oshima and Imahori, ; Williams et al., ) and 80°C (Swarup et al., ). The oxidative dissolution of pyrite (FeS 2) by pure and mixed cultures of moderately thermophilic acidophiles was studied in shake flask cultures and in pH‐controlled bioreactors, incubated at 45°s combinations of seven eubacteria (a Leptospirillum sp. (MT6), Acidimicrobium ferrooxidans, Acidithiobacillus caldus, an Alicyclobacillus sp. (Y), and three Sulfobacillus spp.) and.

  (). Seasonal variations in number of acidophilic iron-oxidizing bacteria and iron oxidation in the river contaminated with acid mine water. Soil .   Moderate thermophiles have temperature optima between 40°C and 60°C and, with the current sole exception of the sulfur‐reducing euryarchaeote Thermoplasma, are also bacteria. In contrast, all known sulfur‐metabolizing extreme thermophiles (temperature optima of > 60°C) are crenarchaeotes, apart from the sulfur‐oxidizing autotrophic.

"highlights current areas of research" from IFIS "a solid and critical review of the impact that extremophiles have in biotechnology. It discusses the adaptation of thermophilic, psychrophilic, acidophilic, and radiation-resistant microorganisms in their respective habitats the book offers newcomers to the biotechnology industry a good overview and a simple introduction to the subject. The members of the genus are acidophilic Gram-negative rods, motile by one or more flagella and comprise both mesophiles and moderate thermophiles. All four species are autotrophs capable of growth utilising inorganic compounds such as reduced inorganic sulphur compounds (RISCs) as sole energy substrate.


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Development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles by F. Elizabeth Gibson Download PDF EPUB FB2

Gibson, Acidophilic moderate thermophiles book. Elizabeth () The development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles. PhD thesis, University of : F. Elizabeth Gibson. The development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles Author: Gibson, F.

Elizabeth ISNI: This work describes initial attempts to develop a host:vector system for the moderate thermophiles. The bacteria were found to be microaerophilic and a pour plate technique and filter disc assay Author: F.

Elizabeth Gibson. The development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles. By F. Elizabeth Gibson. Get PDF (7 MB) Abstract. Acidophilic, moderately thermophilic, Gram positive bacteria which are able to oxidize iron and solubilize sulphide ores are likely to be of industrial importance for the leaching of metals from mineral Author: F.

Elizabeth Gibson. The development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles. Thesis (Thesis) Find all citations by this author (default). The Development of Genetic Systems For Iron-Oxidizing, Acidophilic Moderate Thermophiles. by F. Elizabeth Gibson, (Hons.) (CNAA) This thesis is presented for the Degree of Doctor of Riilosophy, in the Department of Biological Sciences, Ifoiversity of Warwick.

July Most of the moderately thermophilic, acidophilic iron-oxidizing bacteria which have been isolated required a source of reduced sulphur for growth on iron.

One isolate (strain ALV) utilized sulphate as the sole source of sulphur. All of the isolates were capable of chemolitho-heterotrophin growth on iron in the presence of yeast extract.

Autotrophic growth has been confirmed in all strains except one previously described, but now re-isolated, moderate thermophile (TH3). INTRODUCTION Early studies of moderately thermophilic, acidophilic, iron-oxidizing bacteria demonstrated growth with iron and mineral sulphide oxidation in the presence of yeast extract [].

Burton NP, Gibson FE, Murrell JC et al. Development of genetic systems for moderately thermophilic, mineral sulfide-oxidizing bacteria. In: Alberghina L, Frontali L, Sensi P, eds. Proceedings of the 6th European Congress on Biotechnology.

Amsterdam: Elsevier – Google Scholar. archaea are acidophilic, the anaerobic thermophilic bacteria Among the moderate thermophiles and thermotolerant. genetic tools for use with these organisms portends for. ADVERTISEMENTS: The following points highlight the six important groups of extremophiles.

The groups are: 1. Acidophiles 2. Alkalophiles 3. Halophiles 4. Psychrophiles 5. Thermophiles and Hyperthermophlies 6. Barophiles. Group # 1. Acidophiles: Most natural environments on the earth are essentially neutral, having pH between 5 and 9. Only a few microbial species can grow at [ ].

“Thiobacillus prosperus” is a halotolerant mesophilic acidophile that gains energy through iron and sulfur oxidation.

Its physiology is poorly understood. Here, we describe the principal genomic features of the type strain of T. prosperus, DSM This is the first public genome sequence of an acidophilic halotolerant bacterium. This indicated that Sulfobacillus acidophilus cooperating with other moderate thermophiles could obtain high copper extraction in the bioleaching of chalcopyrite ores.

4 Conclusions 1) A moderately thermophilic acidophilic iron-oxidizing bacterium ZW-1 is isolated form Dexing mine, Jiangxi Province, China.

Acidophilic iron-oxidizing bacteria are generally considered the most significant microorganisms in the biological processing of sulfide ores (“biomining”) in which the accelerated oxidative dissolution of sulfidic minerals (e.g., pyrite, arsenopyrite, and chalcopyrite) solubilizes (e.g., copper) or.

Ecology of Thermophiles Ecology of Extreme Thermophilic Archaea Ecology of Extreme Thermophilic Bacteria Biotechnology of Thermophiles Acidophiles Acidophilic Ecosystems Alkaliphiles Alakalyphilic Ecosystems Biotechnology of Acidophilic and Alkaliphilic.

In addition to these mesophilic iron oxidizing bacteria, the existence of moderately thermophilic iron oxidizing bacteria has been reported [[]].

These moderate thermophiles have an optimum growth temperature of ≈ 50 °C and derive energy for growth from the oxidation of ferrous iron or metal sulfides.

A legacy of coal mining in the Appalachians has provided a unique opportunity to study the ecological niches of iron-oxidizing microorganisms.

Mine-impacted, anoxic groundwater with high dissolved-metal concentrations emerges at springs and seeps associated with iron oxide mounds and deposits.

These deposits are colonized by iron-oxidizing microorganisms that in some cases efficiently remove. Anaerobic growth of iron-oxidizing moderate thermophiles.

All five of the acidophilic bacteria used in the present study grew well on overlaid ferrous iron solid media (6); however. Extremely acidophilic iron-oxidizing Gram-positive bacteria comprise species within the phyla Firmicutes and Actinobacteria. Here, we report the Mb draft genome of Acidithrix ferrooxidans Py-F3, which was isolated from a stream draining an abandoned copper mine and proposed as the type species of a new genus of Actinobacteria.

Two novel extremely acidophilic, iron-oxidizing actinobacteria were isolated, one from a mine site in North Wales, UK (isolate T23T), and the other from a geothermal site in Yellowstone National Park, Wyoming, USA (YT). These new actinobacteria belong to the subclass Acidimicrobidae, and in contrast to the only other classified member of the subclass (Acidimicrobium ferrooxidans), both.

Acidophiles are life-forms that grow preferentially in natural or man-made environments where the pH is well below seven. Together with other categories of extremophiles, they have greatly expanded our knowledge of the diversity of life, our understanding on how microorganisms can adapt to seemingly hostile situations, and provided scenarios for the possibility that life-forms may be found.

JOHNSON ETAL. weeks. Bacterial colonies were then divided into those whichwere ferric iron encrusted (i.e., iron-oxidizing bacte- ria) and those which were not (i.e., acidophilic hetero- trophs). Colonies, whichweregenerally.This Special Issue is focused on the genetics and genomics of acidophiles and their viruses and seeks studies that focus on comparative genomics, genetic systems, the genetic/genomic mechanisms of acidophilic adaptations and/or microbe-mineral interactions, genomic evolution, and genetic exchange, among other interrelated topics.Norris PR, Barr DW () Growth and iron oxidation by acidophilic moderate thermophiles.

FEMS Microbiol Lett – CrossRef Google Scholar Norris PR, Davis-Belmar CS, Brown CF, Calvo-Bado LA () Autotrophic, sulfur-oxidizing actinobacteria in acidic environments.